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Key DE-miRNA validation by qRT-PCR. (A) logFC value of MTB-1458-infected cells vs. uninfected cells according to sequencing data. (B) THP-1 cells were infected with MTB-1458 at a MOI of 10 for 12 h. Cells were washed twice and a medium containing gentamycin (100 μg/mL) was added. At 6 and 24 hpi, total RNA was collected. The expression level of DE-miRNAs was measured by qRT-PCR and normalized with U6 as an internal reference.
