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. 2021 Dec 29;23(1):382. doi: 10.3390/ijms23010382

Figure 6.

Figure 6

BHLHE40-miR-378d-BHLHE40 regulation axis verification. (A) THP-1 cells were infected with MTB-1458 at a MOI of 10 for 12 h. Cells were washed twice and a medium with gentamycin (100 μg/mL) was added. Thereafter, total RNA was collected at 6 and 24 hpi. BHLHE40 expression level was evaluated by qRT-PCR. (B) The binding site of miR-378d with BHLHE40 3′-UTR mRNA or mutant of BHLHE40 3′-UTR mRNA. (C) Dual-luciferase reporter assay was executed in 293T cells. The psiCHECK2, psiCHECK2-BHLHE40-wild, and psiCHECK2-BHLHE40-mut plasmids were cotransfected with NC mimic or miR-378d mimic. The relative luciferase activity means Renilla to firefly ratio (Rluc/Fluc) in miR-378d mimic or NC mimic. KD represents knockdown. “***” represents p < 0.001, “+” represents presence, “−” represents absence.