Figure 2.

Endothelial cell proliferation elicited by SRC-IgG via ERK1/2–Ets-1 signaling. Non-stimulated cells (Ctrl) were used as reference when natural ligands were included, whereas Ctrl-IgG served as reference when only IgG were used. HMEC-1 were stimulated for 24 h with either natural ligands, Ctrl- or SRC-IgG, and specificity was assessed via two-hour pre-incubation with corresponding receptor inhibitors (a) (left and right) or cRaf1 inhibitor (b). (c) Abolition of Ets-1 translational regulation by shRNA following six-hour HMEC-1 stimulation. Ctrl shRNA corresponds to a mix of three control shRNA plasmids. Blots were over-exposed to better appreciate the decrease in the protein level. (d) Decrease in SRC-IgG induced endothelial cell proliferation by Ets-1 knockdown. (a–c) n = 4, (d) 7 ≤ n ≤ 11; representative blots are shown. * p < 0.05.