Figure 4.

HopAU1 activates immunity responses in Nicotiana benthamiana. (A) ROS accumulation and callose deposition in N. benthamiana leaves by transiently expressing HopAU1-GFP and GFP control, respectively; Photographs were taken 2 days after infiltration; Bars = 50 μm. (B) qRT-PCR analysis of hypersensitive-response-speicfic marker genes and defense-related marker gene in N. benthamina. Transcript levels of candidate genes were normalized to that of NbActin and GFP was used as control. The statistical data were visualized with Mean ± standard errors (SEs) using Student’s t-test (***, p < 0.001).