Figure 5.

Confirmation of the interaction between HopAU1 and CaS. (A) BiFc analysis of HopAU1 and CaS in N. benthamiana. Photographs were taken after 2 days post HopAU1 tagged cYFP and CaS tagged nYFP co-expression in N. benthamiana leaves. Bars = 20 µm. (B) Co-immunoprecipitation (Co-IP) was performed for confirming the interaction. HopAU1-GFP and CaS-mCherry (or GFP and Cas-mCherry) were transiently co-expressed in N. benthamiana. Total protein was extracted and western blotting with anti-GFP or anti-mCherry antibodies, respectively. The bound proteins were affinity purified by GFP-Trap Immunomagnetic beads and then detected with the indicated antibodies.