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. 2021 Dec 28;23(1):331. doi: 10.3390/ijms23010331

Figure 4.

Figure 4

miR-539 and miR-582 regulate genes involved in angiogenesis-related biological processes in ECs and SMCs. (a,d) Transcriptomic analysis of ECs transfected with pre-miR-539 (a) and pre-miR-582 (b) and SMCs transfected with pre-miR-539 (c) and pre-miR-582 (d). Pie chart shows all significant regulated genes (p < 0.05) analyzed by RNA sequencing with the number of upregulated genes colored in red while downregulated genes are labeled in blue. Light blue shows number of potential microRNA targets. (e–h) Validation of some putative targets by qPCR. (e) ECs were transfected with pre-miR-control or pre-miR-539. (f) ECs were transfected with pre-miR-control or pre-miR-582. (g) SMCs were transfected with pre-miR-control or pre-miR-539. (h) SMCs were transfected with pre-miR-control or pre-miR-582. All data are the mean ± SD (n = 3 independent experiments). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the respective control.