Figure 3.

Poloxamers increase primary rat osteoblast viability at 72 h in the presence or absence of LF. (a) Representative phase images of osteoblasts. Scale bar = 200 µm. (b) Osteoblast viability results at 24 and 72 h. Results from three biological experiments were pooled together (n = 3 for each experiment). The cells-only group had inserts hanging on the well without any gel added in the inserts. The 200-µL poloxamers gel was added to each insert. Data shown are the mean ± SEM; two-way ANOVA with post hoc Dunnett’s test, * p < 0.05 versus the cells-only group (control) at that time point.