Table 4.
The major peptide components of the water-soluble extracts of (a) skimmed milk inoculated with individual strains of LAB consortium; (b) dried fresh cheese fermented by LAB consortium, control cheese (fermented by DSM CT-203 starter culture), their separated whey, and milk before starter inoculation. Peptides were identified with LC-MALDI-TOF/TOF. Values are normalized data obtained after targeted LC-MRM-MS analysis.
| (a) | ||||||||
| Peptide Sequence/Protein Source | L. plantarum | L. brevis | L. fermentum | E. faecium | ||||
| D13 (24 h) | D13 (48 h) | D6 (24 h) | D6 (48 h) | D12 (24 h) | D12 (48 h) | ZGZA7-10 (24 h) | ZGZA7-10 (48 h) | |
|---|---|---|---|---|---|---|---|---|
| WMHQPHQPLPPT/ Beta-casein |
248.75± 114.43 | 131.14 ± 102.02 | 239.13 ± 98.18 | 168.66 ± 99.33 | 138.90 ± 26.54 | 195.07 ± 55.73 | 3.09 ± 2.37 | n.d. |
| SWMHQPHQPLPPT/ Beta-casein |
45.83 ± 17.58 | 23.53 ± 23.18 | 24.64 ± 8.35 | 16.50± 11.51 | 8.36 ± 3.29 | 55.74 ± 19.51 | n.d. | n.d. |
| SQSKVLPVPQKAVPYPQ/ Beta-casein |
n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| YQEPVLGPVR/ Beta-casein |
n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| RDMPIQAF/ Beta-casein |
n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| HQPHQPLPPTVMFPPQ/ Beta-casein |
n.d. | n.d. | n.d. | n.d. | n.d. | 7.53 ± 3.01 | n.d. | n.d. |
| TKVIPYVRYL/ Alpha-S2-casein |
4.19 ± 1.48 | 1.17 ± 0.86 | 2.39 ± 2.23 | 2.67 ± 0.62 | n.d. | n.d. | 6.40 ± 1.62 | 4.01 ± 3.38 |
| VLGPVRGPFP/ Beta-casein |
36.96 ± 21.31 | 89.09 ± 123.62 | 39.27 ± 14.51 | 26.89 ± 17.50 | 89.95 ± 32.41 | 396.76 ± 109.19 | 1.08 ± 0.77 | n.d. |
| WIQPKTKVIPYVRYL/Alpha-S2-casein | 8.36 ± 2.16 | 5.92 ± 3.46 | 10.13 ± 3.77 | 6.95 ± 4.49 | n.d. | n.d. | n.d. | n.d. |
| APSFSDIPNPIGSENSE/ Alpha-S1-casein |
n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| LVYPFPGPIHNSLPQN/ Beta-casein |
15.02 ± 3.00 | 15.60 ± 3.33 | 14.13 ± 2.43 | 8.28 ± 8.27 | 10.14 ± 2.74 | 11.17 ± 0.85 | n.d. | n.d. |
| LVYPFPGPIHNSLPQ/ Beta-casein |
3.59 ± 2.24 | 2.59 ± 2.02 | 3.32 ± 3.76 | 3.54 ± 0.30 | 6.38 ± 7.09 | 10.58 ± 3.18 | n.d. | n.d. |
| VYPFPGPIPN/ Beta-casein |
n.d. | n.d. | 1.27 ± 0.81 | n.d. | n.d. | n.d. | n.d. | n.d. |
| QEPVLGPVRGPFPIIV/ Beta-casein |
9.70 ± 1.41 | 4.61 ± 3.60 | 5.65 ± 2.93 | 3.32 ± 2.15 | 2.11 ± 1.94 | 5.62 ± 0.54 | 2.64 ± 0.67 | 1.65 ± 1.39 |
| YQEPVLGPVRGPFPIIV/ Beta-casein |
95.53 ± 17.96 | 74.71 ± 32.21 | 49.54 ± 14.87 | 26.41 ± 20.03 | 52.71 ± 31.15 | 65.61 ± 8.68 | 28.48 ± 7.49 | 17.98 ± 14.83 |
| FVAPFPEVFG/ Alpha-S1-casein |
n.d. | 1.03 ± 0.61 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| LYQEPVLGPVRGPFPIIV/ Beta-casein |
27.31 ± 13.59 | 7.24 ± 4.11 | 20.43 ± 17.83 | 12.88 ± 10.89 | 11.61 ± 9.29 | 12.41 ± 2.45 | 3.62 ± 3.06 | 3.34 ± 0.40 |
| LLYQEPVLGPVRGPFPIIV/ Beta-casein |
3.22 ± 1.82 | n.d. | 3.20 ± 4.15 | n.d. | 1.05 ± 0.73 | 1.07 ± 0.13 | 1.13 ± 1.59 | n.d. |
| (b) | ||||||||
|
Peptide Sequence/
Protein Source |
RT | Control Cheese | Control Whey | Cheese with LAB Consortium | Whey of LAB Consortium | Control | ||
| WMHQPHQPLPPT/ Beta-casein |
8.953 | 1.67 ± 0.72 | n.d. | 13.08 ± 0.89 | 1.14 ± 0.11 | n.d. | ||
| SWMHQPHQPLPPT/ Beta-casein |
9.446 | 20.41 ± 2.79 | 46.92 ± 3.82 | 132.39 ± 18.65 | 22.10 ± 8.07 | n.d. | ||
| SQSKVLPVPQKAVPYPQ/ Beta-casein |
10.069 | 56.48 ± 28.21 | 46.01 ± 7.46 | 90.90 ± 3.32 | 35.34 ± 7.67 | n.d. | ||
| YQEPVLGPVR/ Beta-casein |
10.210 | 2.84 ± 0.95 | n.d. | 7.69 ± 0.29 | n.d. | n.d. | ||
| RDMPIQAF/ Beta-casein |
11.373 | 51.08 ± 12.79 | 25.32 ± 0.31 | 108.89 ± 5.37 | 7.70 ± 0.21 | n.d. | ||
| HQPHQPLPPTVMFPPQ/ Beta-casein |
11.582 | n.d. | n.d. | 9.55 ± 1.80 | 4.25 ± 0.75 | n.d. | ||
| TKVIPYVRYL/ Alpha-S2-casein |
11.723 | 49.02 ± 12.40 | 69.00 ± 11.30 | 35.52 ± 6.36 | 12.59 ± 5.62 | 40.70 ± 6.50 | ||
| VLGPVRGPFP/ Beta-casein |
12.083 | n.d. | n.d. | n.d. | n.d. | n.d. | ||
| WIQPKTKVIPYVRYL/ Alpha-S2-casein |
12.172 | n.d. | n.d. | n.d. | n.d. | n.d. | ||
| APSFSDIPNPIGSENSE/ Alpha-S1-casein |
12.506 | 6.54 ± 0.40 | n.d. | 20.89 ± 0.21 | n.d. | n.d. | ||
| LVYPFPGPIHNSLPQN/ Beta-casein |
12.917 | n.d. | n.d. | 1.74 ± 2.42 | n.d. | n.d. | ||
| LVYPFPGPIHNSLPQ/ Beta-casein |
13.279 | 2.77 ± 0.38 | 5.94 ± 0.78 | 1.49 ± 2.08 | 1.24 ± 0.36 | n.d. | ||
| VYPFPGPIPN/ Beta-casein |
13.373 | 1.69 ± 0.67 | 1.85 ± 0.53 | 7.66 ± 0.33 | n.d | n.d. | ||
| QEPVLGPVRGPFPIIV/ Beta-casein |
15.696 | 19.27 ± 1.83 | 125.57 ± 19.77 | 25.93 ± 2.05 | 77.66 ± 24.10 | n.d. | ||
| YQEPVLGPVRGPFPIIV/ Beta-casein |
15.949 | 1432.50 ± 228.82 | 361.51 ± 33.00 | 682.92 ± 57.39 | 97.39 ± 30.35 | 11.25 ± 6.08 | ||
| FVAPFPEVFG/ Alpha-S1-casein |
16.093 | 37.60 ± 3.70 | n.d. | 35.79 ± 0.70 | n.d. | n.d. | ||
| LYQEPVLGPVRGPFPIIV/ Beta-casein |
16.250 | 47.63 ± 10.13 | 6.54 ± 1.20 | 20.24 ± 0.44 | 2.64 ± 0.32 | 5.82 ± 2.54 | ||
| LLYQEPVLGPVRGPFPIIV/Beta-casein | 16.743 | 9.05 ± 3.15 | 8.37 ± 1.31 | n.d. | n.d. | n.d. | ||
n.d.—not detected.