Figure 2.

Top: Consecutive live cell fluorescence images of microtubules in COS-7 cells labeled with mGarnet2 using confocal microscopy and STED nanoscopy. Bottom: Normalized fluorescence intensity of the images as a function of the frame number upon confocal (black, violet) and STED (grey, light violet) imaging of live COS7 cells labeled with mGarnet (black, grey), and mGarnet2 (violet, light violet). Pixel dwell time: 40 $\mathsf{\mu }\mathrm{s}$. Excitation laser wavelength: 640 nm, Excitation laser power: 12.5 $\mathsf{\mu }\mathrm{W}$, STED laser wavelength: 780 nm, STED laser power: 36$\mathrm{mW}$. (Reproduced from Ref. [31] with permission from the Royal Society of Chemistry).