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. 2021 Dec 22;27(1):36. doi: 10.3390/molecules27010036

Figure 6.

Figure 6

Effect of (3β,5α,22E)-ergost-22-en-3-ol (compound 4) on the protein expression of phospho-extracellular-signal-regulated kinase (p-ERK), ERK, phospho-phosphatidylinositol 3-kinase (p-PI3K), PI3K, phospho-Akt (p-Akt), Akt, phospho-estrogen receptor α (p-ERα), and ERα in MCF-7 cells. (A) Protein expression levels of p-PI3K, PI3K, p-Akt, Akt, p-ERα, ERα, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in MCF-7 cells treated or untreated with 25 µM, 50 µM, and 100 µM compound 4 for 24 h. (B–E) Bar graph presents the densitometric quantification of Western blot bands. * Significant difference between cells treated with compound 4 and the untreated cells (n = 3 independent experiments, p < 0.05, Kruskal–Wallis nonparametric test). Data are the mean ± SEM.