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. 2021 Dec 22;27(1):36. doi: 10.3390/molecules27010036

Figure 7.

Figure 7

Effect of (3β,5α,22E)-ergost-22-en-3-ol (compound 4) in the absence or presence of ICI 182,780 (ICI) on the protein expression of phospho-extracellular-signal-regulated kinase (p-ERK), ERK, phospho-phosphatidylinositol 3-kinase (p-PI3K), PI3K, phospho-Akt (p-Akt), Akt, phospho-estrogen receptor α (p-ERα), and ERα in MCF-7 cells. (A) Protein expression levels of p-PI3K, PI3K, p-Akt, Akt, p-ERα, ERα, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in MCF-7 cells treated or untreated with concentrations of 100 µM compound 4, either with or without 100 nM ICI 182,780 (ICI) for 24 h. (B–E) Bar graph presents the densitometric quantification of Western blot bands. * Significant difference between cells treated with compound 4 and the untreated cells. # Significant reduction in co-treatment with ICI compared to treatment with compound 4 alone (n = 3 independent experiments, p < 0.05, Kruskal–Wallis nonparametric test). Data are represented as mean ± SEM.