Skip to main content
. 2021 Dec 24;27(1):97. doi: 10.3390/molecules27010097

Figure 2.

Figure 2

Quantitative and normalized profiles of Ctf4-GFP and Ctf4-GFP;dia2Δ throughout the cell cycle. (a) Averaged total fluorescence intensity (mean intensity × area) of Ctf4-GFP and Ctf4-GFP;dia2Δ. (b) Averaged mean fluorescent intensity of the emitted GFP signal from the nucleus of Ctf4-GFP and Ctf4-GFP; dia2Δ. (c) Averaged area of the nucleus of Ctf4-GFP and Ctf4-GFP;dia2Δ yeast cells. For (a–c), the minimum value for each graphic is normalized to 0. (d–f) The data from (a–c) are normalized between 0 to 1 and are represented in (d–f), respectively. For each time point, the background was subtracted and maximum intensity projection function of 11 z-stacks was applied. A total of 10 cells with averaged durations of the cell cycle for each strain were selected and the standard error of mean is represented. Representative images of the two strains demonstrate the measured region of interest (ROI).