Figure 2.
Inhibition of OSM levels by each inhibitor. dHL-60 cells (5 × 105) were pretreated with each inhibitor for 1 h and then stimulated with GM-CSF (5 ng/mL) for 4 h. OSM levels were measured with the ELISA method. B—PBS-treated and unstimulated cells; C—PBS-treated and GM-CSF-stimulated cells; Wort—50 μM of wortmannin-treated and GM-CSF-stimulated cells; MK 2206—50 μM of MK 2206-treated and GM-CSF-stimulated cells; PDTC—100 μM of PDTC-treated and GM-CSF-stimulated cells. Data are presented as the mean ± S.E.M. of three independent experiments. * p < 0.05 vs. the PBS-treated and GM-CSF-stimulated cells.