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. 2021 Dec 29;27(1):204. doi: 10.3390/molecules27010204

Figure 4.

Figure 4

(A) Calculated optimization plot (red data/red current line) using the Minitab 10 software profile optimizer tool for Man-BSA-CS-NPs; (B) TEM images of optimized Man-BSA-CS-NPs (scale bar: 100 nm); (C) DLS image of optimized Man-BSA-CS-NPs. (Preparation condition of optimized Man-BSA-CS-NPs: 3 mg/mL Man, 24 h co-mixing time and 250 μg BSA; the concentration of CS solution was 1 mg/mL; CS solution was kept at 5 mL with pH 4.0; the volume ratio of CS solution: TPP solution was kept at 5:2).