Table 5.
The 23 full factorial design of experimental sites for Man-BSA-CS-NPs (n = 3). (a) Values of levels of process parameters. (b) Experimental design and experimental values of particle size, PDI and zeta potential.
| (a) | ||||||
| Variable Factor | Low Level | Mid-Level | Low Level | |||
| Concentration of Mannose (Man) X1 (mg/mL) | 1 | 2 | 3 | |||
| The blending time of CS and Mannose (Co-Mixing Time) X2 (h) | 6 | 12 | 24 | |||
| Amount of bovine serum albumin (BSA) X3 (μg) | 250 | 500 | 1000 | |||
| (b) | ||||||
| Run |
Man
(mg/mL) |
Mixing Time
(h) |
BSA
(μg) |
Particle Size
(nm) |
PDI |
Zeta
(mV) |
| 1 | 1 | 6 | 250 | 189.1 (±2.3) |
0.324 (±0.044) | 33.8 (±0.5) |
| 2 | 1 | 6 | 500 | 176.9 (±1.0) |
0.287 (±0.015) | 33.6 (±0.9) |
| 3 | 1 | 6 | 1000 | 192.4 (±2.2) |
0.272 (±0.008) | 37.7 (±0.7) |
| 4 | 1 | 12 | 250 | 155.1 (±1.3) |
0.218 (±0.017) | 32.9 (±0.8) |
| 5 | 1 | 12 | 500 | 150.4 (±3.0) |
0.200 (±0.011) | 30.8 (±1.1) |
| 6 | 1 | 12 | 1000 | 141.8 (±3.0) |
0.211 (±0.005) | 31.6 (±1.1) |
| 7 | 1 | 24 | 250 | 183.3 (±0.9) |
0.170 (±0.007) | 29.0 (±0.7) |
| 8 | 1 | 24 | 500 | 172.8 (±2.3) |
0.223 (±0.009) | 34.7 (±1.0) |
| 9 | 1 | 24 | 1000 | 189.9 (±2.4) |
0.260 (±0.013) | 34.3 (±1.0) |
| 10 | 2 | 6 | 250 | 161.6 (±4.1) |
0.211 (±0.016) | 32.9 (±0.5) |
| 11 | 2 | 6 | 500 | 164.3 (±4.9) |
0.200 (±0.015) | 31.4 (±0.8) |
| 12 | 2 | 6 | 1000 | 172.7 (±3.7) |
0.197 (±0.012) | 30.4 (±0.5) |
| 13 | 2 | 12 | 250 | 161.7 (±5.4) |
0.246 (±0.004) | 34.2 (±0.3) |
| 14 | 2 | 12 | 500 | 161.8 (±2.6) |
0.234 (±0.016) | 33.6 (±0.4) |
| 15 | 2 | 12 | 1000 | 169.7 (±4.3) |
0.258 (±0.008) | 30.9 (±0.6) |
| 16 | 2 | 24 | 250 | 179.0 (±2.6) |
0.224 (±0.019) | 32.7 (±0.4) |
| 17 | 2 | 24 | 500 | 141.3 (±3.6) |
0.190 (±0.024) | 32.9 (±0.4) |
| 18 | 2 | 24 | 1000 | 158.4 (±4.2) |
0.189 (±0.009) | 36.4 (±1.5) |
| 19 | 3 | 6 | 250 | 153.3 (±4.3) |
0.211 (±0.026) | 31.7 (±0.9) |
| 20 | 3 | 6 | 500 | 172.1 (±5.3) |
0.218 (±0.004) | 30.2 (±1.5) |
| 21 | 3 | 6 | 1000 | 161.4 (±4.0) |
0.228 (±0.017) | 30.7 (±1.2) |
| 22 | 3 | 12 | 250 | 161.8 (±2.5) |
0.224 (±0.018) | 33.7 (±0.4) |
| 23 | 3 | 12 | 500 | 150.9 (±5.2) |
0.233 (±0.015) | 32.6 (±0.9) |
| 24 | 3 | 12 | 1000 | 160.6 (±2.7) |
0.232 (±0.003) | 32.9 (±0.6) |
| 25 | 3 | 24 | 250 | 142.3 (±2.9) |
0.183 (±0.019) | 32.9 (±0.4) |
| 26 | 3 | 24 | 500 | 177.0 (±5.2) |
0.248 (±0.013) | 33.3 (±0.6) |
| 27 | 3 | 24 | 1000 | 170.1 (±3.7) |
0.240 (±0.019) | 31.6 (±0.7) |