Figure 5.

Data quantifying anti-biofilm activity of dioscin against H. pylori J99 after 24-h incubation under flow conditions. The flow conditions were generated using the Bioflux 1000 system. The MIC, ½ × MIC, and ¼ × MIC had values of 64 µg/mL, 32 µg/mL, and 16 µg/mL, respectively. Control samples were bacteria not exposed to any concentration of dioscin. Viability was defined as the ratio of green to red fluorescence of bacterial cells stained with the LIVE/DEAD kit, while the development of biofilm was verified by determining the degree of capillary coverage (both parameters were determined using the Bioflux Montage software). The study was performed with triplicate biological replications (n = 3). The p-value represented by ns, ***, and **** is equal to > 0.05, < 0.001, and < 0.0001, respectively.