Table 3.
Numerical values showing interactions between dioscin and the three classically used antibiotics (clarithromycin [CLR], metronidazole [MTZ], and levofloxacin [LEV]) in antibacterial activity against H. pylori J99. Interactions were determined using the checkerboard assay.
| Tested Combination |
MIC [µg/mL] | FICI (Outcome) |
|||||
|---|---|---|---|---|---|---|---|
| Dioscin | Antibiotic | ||||||
| Alone | Combination | Fold Change | Alone | Combination | Fold Change | ||
| Dioscin + CLR |
64 | 32 | 2 | 0.025 | 0.006 | 4 | 0.75 (additive) |
| Dioscin + MTZ | 64 | 16 | 4 | 1 | 0.5 | 2 | 0.75 (additive) |
| Dioscin + LEV |
64 | 32 | 2 | 1 | 0.5 | 2 | 1.0 (additive) |
The fractional inhibitory concentration index (FICI) values of ≤ 0.5, > 0.5 to ≤ 1, and > 1 to ≤ 4 were expressed as synergistic, additive, and neutral, respectively.