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. 2021 Dec 27;14(1):104. doi: 10.3390/nu14010104

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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MLB supplementation attenuated HFD-induced muscle atrophy. At the end of the experiment, the skeletal muscles, namely the gastrocnemius (GC), extensor digitorum longus (EDL), and soleus (SOL) muscles, were removed and weighed. The ratios of the weight of the GC, SOL, and EDL muscles to the body weight are depicted (A–C). The representative hematoxylin and eosin (H&E) staining of GC muscles from each group are presented (D), and the relative muscle cross-sectional area (CSA) data are illustrated (E). Measurement of variance coefficients of the fiber size in the cross-sectional samples of three groups using Feret’s diameter as the geometrical parameter (F). Values are expressed as the mean ± SD. * p < 0.05 vs. control group. # p < 0.05 vs. HFD group. n = 8.