Crystal structure of TsaBgl and comparison with other Bgls. (a) Cartoon representation of the TsaBgl structure with a Tris molecule bound to the active site in the center of the TIM-barrel fold. (b) B-Factor putty representation of TsaBgl. The β8-α14 loop is more flexible (green to red) than other regions (blue). (c) Close-up view of the superimposition of β8-α14 loop region of TsaBgl with that of HorBglB (PDB code: 4PTX), EanBglB (5DT7), CceBglB (3AHX), and TmaBgl (2J79). (d) Partial sequence alignments of TsaBgl (UniProt code: I3VXG7) with HorBglB (B8CYA8), EanBglB (K0A8J9), CceBglB (Q53EH2), and TmaBgl (Q08638).