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. 2022 Jan 4;27(1):290. doi: 10.3390/molecules27010290

Table 3.

Comparison of kinetic parameters across the GH family 1 Bgls a.

Enzyme Km (mM) kcat (s−1) kcat/Km (mM−1 s−1) Optimal Conditions Reference
TsaBgl 0.36 ± 0.02 18.62 ± 0.32 50.99 ± 2.28 pH 6, 55 °C This study
TthBgl 0.63 ND b ND b pH 6.4, 70 °C [36]
EanBglA 1.07 32.98 30.8 pH 7, 30 °C [37]
CceBglA 0.15 ± 0.01 50.67 ± 1.00 340 ± 27 pH 6, 45 °C [29]
TreBgl2 0.86 ± 0.07 6.91 ± 0.16 8.1 ± 0.8 pH 6, 40 °C [29]
NkoBgl 0.29 ± 0.02 1.39 ± 0.03 4.8 ± 0.4 pH 5.5, 45 °C [29]
TmaBglA 0.38 ± 0.02 452.27 ± 8.25 1210 ± 140 pH 6.2, 90 °C [38]

a Parameters compared here were obtained using pNPG as the substrate. b ND represents “not determined” in the reference.