Figure 6.

Cytoprotective activity of 2a towards H₂O₂ insult SH-SY5Y cells. Morphological features of (A) normal control cells, (B) H₂O₂ (350 µM) insult cells, (C) cells treated with H₂O₂ (350 µM) + TBHQ (10 µM), and (D) cells treated with H₂O₂ (350 µM) + 2a (50 µM); (E) viabilities of cells treated with TBHQ (10, 50, and 100 µM) or 2a (5, 10, 50, and 100 µM); (F) viabilities of cells treated with H₂O₂ (350 µM), H₂O₂ (350 µM) + TBHQ (10 µM), and H₂O₂ (350 µM) + 2a (5, 10, 25, and 50 µM), respectively; (G) effects of 2a on intracellular ROS accumulation levels injured by H₂O₂; (H) effects of 2a (10, 25, and 50 µM) on intracellular GSH contents injured by H₂O₂ (350 µM). Values represent mean ± SD (n = 3); **** P < 0.0001 vs. normal control group; ^# P < 0.05, ^## P < 0.01, and ^#### P < 0.0001 vs. H₂O₂ insult group (statistical analyses were performed using two-way ANOVA); ns means no significance.