TABLE 2.
Cross-reactivity in ELISA between Rhodotorula mannan and sera collected from several spirochetosis patientsa
| Sampleb | Genus of Spirocheta | Serogroup | Detection (avg absorbance)
|
|
|---|---|---|---|---|
| IgM | IgG | |||
| J-1 | Leptospira | Icterohaemorrhagiae | + (0.061)c | + (0.500)c |
| J-2 | Leptospira | Icterohaemorrhagiae | + (0.051)c | + (0.453)c |
| J-3 | Leptospira | Icterohaemorrhagiae | + (0.063)e | + (0.079)c |
| J-4 | Leptospira | Icterohaemorrhagiae | − (0.025) | + (0.131)c |
| J-5 | Leptospira | Icterohaemorrhagiae | − (0.030) | + (0.103)d |
| J-6 | Leptospira | Icterohaemorrhagiae | + (0.063)e | + (0.077)e |
| J-7 | Leptospira | Icterohaemorrhagiae | − (0.047) | + (0.082)e |
| J-8 | Leptospira | Icterohaemorrhagiae | − (0.049) | + (0.102)e |
| J-9 | Leptospira | Icterohaemorrhagiae | − (0.048) | + (0.103) |
| J-10 | Leptospira | Icterohaemorrhagiae | − (0.049) | − (0.049) |
| P-1 | Leptospira | Pyrogenes | + (0.568) | + (0.333) |
| P-2 | Leptospira | Pyrogenes | − (0.039) | + (0.053) |
| P-3 | Leptospira | Pyrogenes | + (0.253) | + (0.050) |
| P-4 | Leptospira | Pyrogenes | + (0.217) | + (0.310) |
| P-5 | Leptospira | Pyrogenes | + (0.271) | + (0.168) |
| L-1 through L-10 | Borrelia | — | − (0.020) | − (0.020) |
| S-1 through S-10 | Treponema | — | − (0.023) | − (0.024) |
a
Unless otherwise indicated, triplicate assays were done by using each 500-fold-dilution serum, and the average data are shown in parentheses. +, positive samples giving an average absorbance higher than twice the control value (0.025); −, negative samples.
b
Sera J-1 to J-10 and sera P-1 to P-5 were collected from Japanese and Filipino patients, respectively.
c
Assayed with 5,000-fold-dilution sera.
d
Assayed with 2,000-fold-dilution sera.
e
Assayed with 1,000-fold-dilution sera.