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. 2021 Dec 25;12(1):51. doi: 10.3390/nano12010051

Figure 5.

Figure 5

Cytotoxicity of AS-AgNPs on the human lung cancer cell line, A549. (A) The dose-dependent effect of AS-AgNPs on the cell viability of human A549 cells was measured by MTT assay after 48 h of treatment. Values are shown as the mean ± SD of three independent experiments. (B) Histological sections of lung tissue stained with (a–c) H&E and (d,e) periodic acid Shiff (PAS) from 3-day post-AS-AgNP instillation. (a) Control mice (HBSS), (b,d) IPA mice, and (c,e) IPA mice treated with AS-AgNPs. Arrows in (b) specify the following: A; degeneration and desquamation of the lining of the respiratory epithelium of the bronchiole; B, dilatation and congestion of blood vessels; C, thickening in the wall of blood vessels; and D, lymphocytic infiltration in tissue. Arrows in (d) and (e) specify the boundaries of fungal lesions. Measurements of inflammatory cytokines (C) MPO, (D) TNF-α, (E) IL-1, and (F) IL-17 at 3 days post-instillation of IPA mice with HBSS or AS-AgNPs. Data are expressed as the means ± SD (n = 8 mice/group). ** p < 0.005 compared to control, non-treated cells.