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. 2022 Jan;192(1):87–103. doi: 10.1016/j.ajpath.2021.10.004

Figure 1.

Figure 1

Loss of hepatic transcription factor EB (TFEB) dampens diethylnitrosamine (DEN)–alcohol–induced hepatocellular carcinoma (HCC) in mice. A: The scheme of DEN-alcohol–Associated HCC model. Male mice at the age of 2 weeks were given one dose of carcinogen DEN (10 mg/kg, intraperitoneally). Eleven weeks later, mice were acclimated to the Lieber-DeCarli liquid control (Ctrl) diet for 1 week, followed by further feeding with the liquid Ctrl or 5% ethanol diet (EtOH) for 24 weeks. B: Representative gross images of livers from Tfeb wild-type (WT) and L-Tfeb knockout (KO) mice from different treatments are shown. C: Summary of tumor formation in Tfeb WT and L-Tfeb KO mice from different treatments. D and E: Maximal tumor diameter (D) and total number of tumors (E) of each mouse from different treatments. F–H: Number of tumors within different size categories of each mouse from different treatments. Data are presented as means ± SEM (D–H). n = 10 to 13; n = 12 Tfeb WT DEN+Ctrl; n = 10 Tfeb WT DEN+EtOH; n = 11 L-Tfeb KO DEN+Ctrl; n = 13 L-feb KO DEN+EtOH. ∗P < 0.05 (one-way analysis of variance with Bonferroni post hoc test). Scale bars = 10 mm (B). Alb-Cre, albumin Cre; CD, control diet; NB, newborn.