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. 2022 Jan;192(1):87–103. doi: 10.1016/j.ajpath.2021.10.004

Figure 3.

Figure 3

Western diet (WD) promotes diethylnitrosamine (DEN)–initiated liver tumorigenesis, which is attenuated by genetic deletion of transcription factor EB (TFEB) in mice. A: The scheme of DEN–Western diet–associated hepatocellular carcinoma model. Male mice at the age of 2 weeks were given one dose of carcinogen DEN (10 mg/kg, intraperitoneally). Two weeks later, mice were fed a control low-fat diet (LFD) or WD containing 42% fat calories and 0.2% cholesterol for 22 or 34 weeks, respectively. B: Representative images of livers from Tfeb wild-type (WT) and L-Tfeb knockout (KO) mice from different treatments are shown. C: Summary of tumor formation in Tfeb WT and L-Tfeb KO mice from different treatments. D: Total number of tumors of each mouse from different treatments. E–G: Number of tumors with different sizes of each mouse from different treatments. Data are presented as means ± SEM (D–G). n = 6 to 14; n = 6 Tfeb WT DEN+LFD 22wks; n = 9 Tfeb WT DEN+WD 22wks; n = 9 Tfeb WT DEN+LFD 34wks; n = 11 Tfeb WT DEN+WD 34wks; n = 8 L-Tfeb KO DEN+LFD 22wks; n = 10 L-Tfeb KO DEN+WD 22wks; n = 13 L-Tfeb KO DEN+LFD 34wks; n = 14 L-Tfeb KO DEN+WD 34wks. ∗P < 0.05 (one-way analysis of variance with Bonferroni post hoc test). Scale bar = 10 mm (B). Alb-Cre, albumin Cre; NB, newborn.