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. 2022 Jan;192(1):87–103. doi: 10.1016/j.ajpath.2021.10.004

Figure 4.

Figure 4

Western diet (WD) increases liver injury in diethylnitrosamine (DEN)–treated mice. Male Tfeb wild-type (WT) and L-Tfeb knockout (KO) mice were treated with a DEN-WD–associated hepatocellular carcinoma (HCC) model. A and B: Serum alanine aminotransferase (ALT) activity (A) and liver/body weight ratio (B) were measured. C: Representative images of hematoxylin and eosin staining are shown. Red arrows denote differentiated HCC cells with increased nuclei/cytoplasm ratio. Black arrows denote steatotic HCC cells. D and E: Total liver lysates were subjected to Western blot analysis. Data are presented as means ± SEM (A and B). n = 6 to 14 (A and B); n = 3 to 4 (D and E). ∗P < 0.05 (one-way analysis of variance with Bonferroni post hoc test). Original magnifications, ×4 (C, left panels); ×10 (C, right panels). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LFD, low-fat diet; TFEB, transcription factor EB.