Figure 1.

Oxidative phenotype plantaris muscle used for SDH staining. A: SDH staining images are representative of the five experimental groups. B: representative of the development of cancer cachexia through % of SDH+ fibers. Distribution through relative frequency (%) of cross-sectional area for SDH+ fibers (C) and SDH− fibers (D). Average cross-sectional area for SDH+ (E) and SDH− fibers (F). G: mRNA abundance for mitochondrial specific markers Cox4i1 and Vdac by using the gastrocnemius muscle. Different letters represent statistical differences at Tukey adjusted with α value set at P ≤ 0.05. n = 4–9 animals/group was used (each n represents average value for each animal across all measured fibers). CSA, cross-sectional area; HT, high tumor; LT, low tumor; PBS, phosphate-buffered saline; SDH, succinate dehydrogenase.