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. 2021 Nov 11;132(1):58–72. doi: 10.1152/japplphysiol.00660.2021

Figure 4.

Figure 4.

Protein degradation and inflammation. A: protein degradation measured by mRNA abundance for Ubc, Atrogin-1, Murf1, and Gadd45a. B: protein content of autophagy-related markers; Beclin-1, LC3, LC3 II/I, and p62 normalized to Ponceau S. C: representative immunoblot image for autophagy-related markers from the same membrane. D: mRNA abundance of autophagy-related markers for Beclin-1, Lc3, and p62. E: mRNA abundance of inflammation markers; IL-6, Tnfα, and NF-κB. Both mRNA and protein contents were analyzed from gastrocnemius muscle. Different letters represent statistical differences at Tukey adjusted with α value set at P ≤ 0.05. n = 10–14 animals/group was used. HT, high tumor; LT, low tumor; PBS, phosphate-buffered saline; Ubc, Ubiquitin C.