. 2021 Nov 11;132(1):58–72. doi: 10.1152/japplphysiol.00660.2021

Table 1.

Characteristics

	PBS	1 wk	2 wk	LT	HT
n	10	11	12	13	14
Body weight, g/mm	1.154 ± 0.014^a,b	1.075 ± 0.013^c	1.125 ± 0.011^b,c	1.197 ± 0.017^a,d	1.287 ± 0.026^e
Tumor weight, mg/mm	N/A	2.376 ± 0.29^a	14.86 ± 2.24^a	43.85 ± 6.37^b	171.2 ± 9.23^c
Body weight–tumor, g/mm	1.154 ± 0.014^a	1.073 ± 0.013^b	1.110 ± 0.011^a,b	1.153 ± 0.016^a	1.115 ± 0.025^a,b
Gastrocnemius, mg/mm	5.542 ± 0.068^a	5.211 ± 0.045^b	5.346 ± 0.06^a,b	5.602 ± 0.073^a	5.369 ± 0.062^a,b
Soleus, mg/mm	0.449 ± 0.01^a	0.403 ± 0.01^a,b	0.418 ± 0.016^a,b	0.43 ± 0.009^a	0.385 ± 0.014^b
Plantaris, mg/mm	0.789 ± 0.016	0.762 ± 0.019	0.753 ± 0.015	0.797 ± 0.02	0.736 ± 0.015
EDL, mg/mm	0.510 ± 0.017	0.488 ± 0.007	0.479 ± 0.013	0.488 ± 0.013	0.475 ± 0.016
TA, mg/mm	2.282 ± 0.057^a	2.174 ± 0.043^a,b	2.151 ± 0.039^a,b	2.213 ± 0.04^a	2.017 ± 0.042^b
Fat, mg/mm	19.23 ± 1.856^a	17.6 ± 1.516^a,b	16.77 ± 1.17^a,b	18.14 ± 1.526^a	12 ± 1.359^b
Heart, mg/mm	6.336 ± 0.18	6.275 ± 0.072	6.22 ± 0.163	6.346 ± 0.154	6.268 ± 0.165
Liver, mg/mm	47.11 ± 1.948^a	46.74 ± 1.994^a	48.85 ± 1.218^a,b	54.78 ± 1.863^b,c	62.3 ± 2.36^d
Lungs, mg/mm	7.695 ± 0.146^a	7.529 ± 0.211^a	7.607 ± 0.228^a	8.146 ± 0.238^a	9.131 ± 0.263^b
Spleen, mg/mm	4.967 ± 0.217^a	4.274 ± 0.186^a	5.471 ± 0.371^a	8.104 ± 0.531^b	19.46 ± 1.123^c
Tibia length, mm	16.57 ± 0.053^a	16.15 ± 0.043^b	16.30 ± 0.079^b,c	16.62 ± 0.057^a,d	16.64 ± 0.049^a,d
Estrogen, pg/mL	67.61 ± 14.85^a	22.43 ± 4.40^b	28.27 ± 4.78^b	32.09 ± 4.19^b	21.77 ± 4.36^b
Progesterone, pg/mL	9.58 ± 1.28^a	5.55 ± 1.55^a,b	3.66 ± 0.63^b	5.02 ± 0.75^b	3.59 ± 1.21^b

Tissue weights are recorded as wet weights. All tissue weights were normalized to their tibia length to account for different body sizes. All values are represented as means ± SE. Different letters represent statistical differences at Tukey-adjusted with α value set at P ≤ 0.05. n = 10–14/group was used. EDL, extensor digitorum longus; HT, high tumor; LT, low tumor; PBS, phosphate-buffered saline; TA, tibialis anterior.