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. 2021 Dec 26;11(1):66. doi: 10.3390/plants11010066

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of EO from A. lanatum on the migration of HepG2 liver cancer cell lines (scratch wound assay). (A) EO inhibits HepG2 human liver cancer cell proliferation and migration. HepG2 cell monolayers were scratched, and cells were supplemented with EO (10 and 25 µg/mL) for 24 h. Migration was determined using an optical microscope (200× magnification) by wound-healing assay. (B) Scratch distance was used to calculate the area of the wound and assess wound closure. The data are shown as the mean ± SD of triplicate values; * p < 0.05 when evaluated with respect to the control (PBS, phosphate-buffered saline).