Figure 4.

Effect of EO from A. lanatum on the inhibition of invasion and angiogenic capacity of HepG2 liver cancer cells. (A) EO inhibits human liver cancer cell migration and invasion. HepG2 liver cancer cell lines were supplemented with EO (10 and 25 µg/mL) and loaded to the upper chambers of matrigel-coated transwells. Invasion was determined by total cell counting. Cells invading the lower chamber after 24 h were counted. (B) The inhibition percentage of invasion was quantified and expressed relative to the control (untreated cells), whose level of invasion was set at 100%. Invading cells quantified using manual counting and values are noted as fold changes, compared to control. The data are shown as the mean ± SD of triplicate values; * p < 0.05 when evaluated against control (PBS, phosphate-buffered saline).