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PCR amplification of the diagnostic 750-bp DNA fragment representing kinetoplastid minicircle DNA. Lanes 1, molecular size marker (200-bp ladder); 2, positive control with 50 ng of purified L. panamensis DNA; 3, negative control (no DNA template); 4 to 15, samples from six CL patients. BDS samples (even-numbered lanes) and MDS samples (odd-numbered lanes) of each patient are directly compared.
