Comparison between the FTIR spectra of the as-received aligners and those immersed for 14 days into staining agents. (a) Erkodur as received (EK) and after exposure to artificial saliva (EK_A), nicotine (EK_B), red wine (EK_C) and coffee (EK_D); (b) Essix Plastic as received (EP) and after exposure to artificial saliva (EP_A), nicotine (EP_B), red wine (EP_C) and coffee (EP_D); (c) Ghost Aligner as received (GA) and after exposure to artificial saliva (GA_A), nicotine (GA_B), red wine (GA_C) and coffee (GA_D); (d) Zendura as received (ZN) and after exposure to artificial saliva (ZN_A), nicotine (ZN_B), red wine (ZN_C) and coffee (ZN_D); (e) Invisalign as received (IL) and after exposure to artificial saliva (IL_A), nicotine (IL_B), red wine (IL_C) and coffee (IL_D).