Table 4.

PCR primers designed to amplify the antioxidant genes catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR) and superoxide reductase (SOD) in the barley plants.

Gene	Sequence	Gi-Number
CAT1	5′-GCGGAAAATGAACAGCTTGC-3′	684945
	5′-CATTCACGGGGAGCATCAAG-3′
CAT2	5′-CGTGGTTGGAAAGAGGGAGA-3′	684947
	5′-ATGCTTGGCTTCACGTTGAG-3′
APX a	5′-GATTCGTCAGTTTGTCCCCG-3′	15080681
	5′-TTTCAGAGGGTCACGAGTCC-3′
GR1 b	5′-TACCGAGGAGCAGGCTATTG-3′	157362216
	5′-TCTTGCTTTGTCAACCCAGC-3′
GR2 c	5′-TCTTTCCGGGGTGAATTCGA-3′	157362218
	5′-ATATGTGCTTCGTCGTGTGC-3′
SOD d	5′-GACTGGCCCTAATGCAGTTG-3′	304651503
	5′-TGGCGTCGTTACAGGTATGA-3′
Control	5′-AAGTACAGTGTCTGGATTGGAGGG-3′	DN182500R533
	5′-TCGCAACTTAGAAGCACTTCCG-3′

a, peroxisome type APX; b, chloroplastic glutathione reductase; c, cytosolic glutathione reductase; d, chloroplast copper/zinc superoxide dismutase.