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. 2021 Dec 25;11(1):55. doi: 10.3390/plants11010055

Table A1.

Mean values (± SE) of antioxidant response (APX, GR, CAT and SOD) and ROS generation (H2O2 and O2−) under two K-supply levels (0.04 and 8 mM K) and two PEG levels (no PEG: -, with PEG: +) in two barley cultivars (Sahin-91 and Milford) 6–25 days after the onset of treatments. The letters (a to d) where present, show intra-cultivar significance of the treatments at the level of (0.05).

DAO K Supply PEG APX GR CAT SOD
(mM K+) (µmol H2O2 g−1 FW min−1) (µmol NADPH g−1 FW min−1) (mmol H2O2 g−1 FW min−1) (U g−1 FW)
Sahin-91
6 0.8 - 1.31 ± 0.19 0.37 ± 0.01 2.01 ± 0.07 128.7 ± 1.3
6 0.04 - 0.72 ± 0.10 0.19 ± 0.01 1.35 ± 0.08 106.8 ± 2.0
10 0.8 - 0.77 ± 0.10 0.34 ± 0.03 1.98 ± 0.07 93.1 ± 3.9
10 0.04 - 1.16 ± 0.06 0.19 ± 00.2 0.91 ± 0.05 88.9 ± 6.8
12 0.8 - 1.53 ± 0.22 0.26 ± 0.02 1.21 ± 0.08 108.0 ± 2.3
12 0.04 - 1.69 ± 0.23 0.27 ± 0.02 0.82 ± 0.06 102.7 ± 6.7
12 0.8 + 1.44 ± 0.09 0.37 ± 0.02 1.61 ± 0.16 103.2 ± 5.2
12 0.04 + 3.64 ± 0.23 0.62 ± 0.06 1.27 ± 0.08 115.6 ± 3.8
14 0.8 - 1.33 ± 0.20 0.24 ± 0.03 1.09 ± 0.08 96.2 ± 6.5
14 0.04 - 1.15 ± 0.08 0.26 ± 0.05 0.82 ± 0.04 107.1 ± 5.9
14 0.8 + 2.86 ± 0.22 0.39 ± 0.04 1.68 ± 0.16 102.5 ± 6.4
14 0.04 + 4.32 ± 0.32 0.68 ± 0.04 0.93 ± 0.09 130.5 ± 4.9
19 0.8 - 1.75 ± 0.23 0.20 ± 0.02 1.22 ± 0.07 91.9 ± 2.4
19 0.04 - 1.81 ± 0.21 0.28 ± 0.03 0.70 ± 0.05 117.0 ± 5.7
19 0.8 + 3.21 ± 0.30 0.81 ± 0.04 1.83 ± 0.20 105.1 ± 7.6
19 0.04 + 6.15 ± 0.36 0.69 ± 0.04 0.49 ± 0.04 131.1 ± 3.4
21 0.8 - 1.53 ± 0.27 0.22 ± 0.02 1.54 ± 0.07 48.5 ± 4.7
21 0.04 - 1.72 ± 0.25 0.26 ± 0.04 0.44 ± 0.08 105.1 ± 5.9
21 0.8 + 1.89 ± 0.12 0.26 ± 0.02 1.88 ± 0.04 67.2 ± 3.2
21 0.04 + 2.06 ± 0.17 0.34 ± 0.02 0.55 ± 0.07 95.3 ± 5.5
25 0.8 - 2.45 ± 0.24 0.16 ± 0.02 1.81 ± 0.13 51.9 ± 5.5
25 0.04 - 2.28 ± 0.25 0.23 ± 0.01 0.41 ± 0.06 98.3 ± 7.8
25 0.8 + 1.73 ± 0.20 0.19 ± 0.02 1.87 ± 0.12 92.0 ± 5.9
25 0.04 + 1.66 ± 0.18 0.26 ± 0.04 0.38 ± 0.01 116.5 ± 1.8
Milford
6 0.8 - 2.33 ± 0.32 0.38 ± 0.02 2.43 ± 0.08 116.6 ± 3.2
6 0.04 - 1.75 ± 0.22 0.32 ± 0.02 1.94 ± 0.10 129.6 ± 1.9
10 0.8 - 1.27 ± 0.16 0.21 ± 0.02 1.50 ± 0.10 122.8 ± 2.7
10 0.04 - 1.79 ± 0.20 0.17 ± 0.03 1.97 ± 0.12 123.4 ± 2.2
12 0.8 - 1.93 ± 0.43 0.25 ± 0.03 1.52 ± 0.09 128.3 ± 3.6
12 0.04 - 1.46 ± 0.12 0.27 ± 0.02 1.59 ± 0.08 142.5 ± 2.4
12 0.8 + 5.71 ± 0.37 0.45 ± 0.04 1.32 ± 0.07 114.1 ± 2.5
12 0.04 + 3.65 ± 0.47 0.53 ± 0.06 2.74 ± 0.17 131.5 ± 1.8
14 0.8 - 1.17 ± 0.15 0.22 ± 0.02 1.59 ± 0.08 117.8 ± 2.5
14 0.04 - 1.77 ± 0.18 0.20 ± 0.02 1.07 ± 0.06 130.0 ± 3.6
14 0.8 + 2.45 ± 0.08 0.46 ± 0.06 2.26 ± 0.09 105.1 ± 3.3
14 0.04 + 9.96 ± 0.50 1.83 ± 0.18 2.50 ± 0.09 150.9 ± 1.2
19 0.8 - 1.67 ± 0.23 0.32 ± 0.02 1.44 ± 0.12 118.8 ± 1.8
19 0.04 - 2.60 ± 0.34 0.27 ± 0.02 1.13 ± 0.09 144.1 ± 3.0
19 0.8 + 2.36 ± 0.17 0.42 ± 0.04 1.98 ± 0.07 114.2 ± 3.1
19 0.04 + 15.32 ± 0.69 2.51 ± 0.13 1.48 ± 0.10 155.7 ± 1.7
21 0.8 - 1.80 ± 0.04 0.13 ± 0.02 1.78 ± 0.13 85.7 ± 6.4
21 0.04 - 2.54 ± 0.32 0.18 ± 0.02 1.33 ± 0.07 103.6 ± 4.4
21 0.8 + 1.37 ± 0.15 0.24 ± 0.02 1.80 ± 0.10 76.0 ± 3.7
21 0.04 + 2.48 ± 0.24 0.14 ± 0.02 1.35 ± 0.09 105.7 ± 4.8
25 0.8 - 2.63 ± 0.33 0.18 ± 0.02 1.77 ± 0.07 104.7 ± 5.7
25 0.04 - 2.49 ± 0.27 0.17 ± 0.02 1.15 ± 0.07 97.7 ± 6.5
25 0.8 + 2.01 ± 0.23 0.18 ± 0.02 1.81 ± 0.11 102.8 ± 4.1
25 0.04 + 1.69 ± 0.19 0.19 ± 0.02 1.15 ± 0.07 118.5 ± 3.0
DAO K Supply PEG H2O2 O2−
(mM K+) (µmol g−1 FW) (nmol g−1 FW min−1)
Sahin-91
6 0.8 - 0.57 ± 0.04 b 0.27 ± 0.07 a
6 0.04 - 1.10 ± 0.11 a 0.31 ± 0.03 a
10 0.8 - 0.52 ± 0.06 b 0.42 ± 0.14 b
10 0.04 - 1.42 ± 0.16 a 2.83 ± 0.29 a
12 0.8 - 0.80 ± 0.05 b 0.58 ± 0.08 c
12 0.04 - 1.61 ± 0.13 a 3.03 ± 0.66 b
12 0.8 + 0.83 ± 0.05 b 5.47 ± 0.48 a
12 0.04 + 1.87 ± 0.14 a 4.71 ± 0.84 a,b
14 0.8 - 0.52 ± 0.04 d 0.77 ± 0.05 c
14 0.04 - 1.00 ± 0.05 b 1.59 ± 0.60 a,b
14 0.8 + 0.77 ± 0.12 c 3.27 ± 0.88 a
14 0.04 + 1.24 ± 0.09 a 3.39 ± 1.09 a
19 0.8 - 0.70 ± 0.12 b 0.39 ± 0.08 b
19 0.04 - 1.46 ± 0.18 a 2.91 ± 0.81 a
19 0.8 + 0.84 ± 0.09 b 2.30 ± 0.66 a,b
19 0.04 + 1.35 ± 0.19 a 2.82 ± 0.78 a
21 0.8 - 0.52 ± 0.10 b 0.30 ± 0.08 a
21 0.04 - 1.02 ± 0.14 a 1.99 ± 0.82 a
21 0.8 + 0.57 ± 0.08 b 0.36 ± 0.09 a
21 0.04 + 1.30 ± 0.15 a 0.40 ± 0.09 a
25 0.8 - 0.49 ± 0.09 b 0.35 ± 0.17 b
25 0.04 - 0.80 ± 0.10 a 3.54 ± 1.80 a
25 0.8 + 0.50 ± 0.04 b 0.70 ± 0.14 b
25 0.04 + 0.62 ± 0.06 a,b 1.18 ± 0.36 b
Milford
6 0.8 - 0.71 ± 0.07 a 0.31 ± 0.05 b
6 0.04 - 0.99 ± 0.11 a 0.78 ± 0.09 a
10 0.8 - 0.61 ± 0.05 a 0.28 ± 0.10 b
10 0.04 - 0.86 ± 0.12 a 2.72 ± 0.57 a
12 0.8 - 0.81 ± 0.05 c 0.52 ± 0.06 c
12 0.04 - 1.14 ± 0.02 b 3.27 ± 0.29 b
12 0.8 + 1.12 ± 0.10 b 1.95 ± 1.25 b,c
12 0.04 + 1.57 ± 0.10 a 6.69 ± 0.55 a
14 0.8 - 0.67 ± 0.04 c 0.68 ± 0.08 b
14 0.04 - 0.76 ± 0.07 b,c 2.89 ± 0.39 b
14 0.8 + 0.96 ± 0.08 b 1.78 ± 0.24 b
14 0.04 + 1.20 ± 0.10 a 6 92 ± 1.01 a
19 0.8 - 0.52 ± 0.07 b 0.70 ± 0.07 b
19 0.04 - 0.93 ± 0.11 a,b 1.66 ± 0.47 b
19 0.8 + 0.93 ± 0.13 a,b 1.76 ± 0.50 b
19 0.04 + 1.02 ± 0.23 a 5.10 ± 0.46 a
21 0.8 - 0.55 ± 0.11 c 0.18 ± 0.06 a
21 0.04 - 0.89 ± 0.10 a,b 0.64 ± 0.12 a
21 0.8 + 0.63 ± 0.07 b,c 0.81 ± 0.30 a
21 0.04 + 0.96 ± 0.08 a 0.24 ± 0.12 a
25 0.8 - 0.37 ± 0.02 b 0.73 ± 0.10 a
25 0.04 - 0.61 ± 0.08 a 0.51 ± 0.08 a
25 0.8 + 0.37 ± 0.04 b 0.70 ± 0.15 a
25 0.04 + 0.72 ± 0.05 a 0.40 ± 0.06 a