Table 3.
Effect of KNO3 priming on total amino acids content, total N, proteins and NR, GDH and GOGAT activities of three species of Medicago sprouts, Values are represented by mean ± standard deviation of at least three independent replicates, Means marked by different letters are significantly different than control at p < 0.05.
| Parameters | Control | KNO3 Priming | ||||
|---|---|---|---|---|---|---|
| M. indicus | M. polymorpha | M. interexta | M. indicus | M. polymorpha | M. interexta | |
| Amino acids (mg·g−1 fw) | ||||||
| Asparagine | 1.64 ± 0.29 a | 1.71 ± 0.38 b | 1.9 ± 0.35 b | 1.79 ± 1.52 a | 1.99 ± 1.73 a | 2.2 ± 1.93 a |
| Glutamine | 1.86 ± 0.41 b | 1.96 ± 0.45 b | 2.17 ± 0.53 b | 3.56 ± 3.32 a | 4.07 ± 3.95 a | 4.27 ± 4.23 a |
| Serine | 1.37 ± 0.04 b | 1.43 ± 0.15 a | 1.59 ± 0.08 b | 2.03 ± 2.16 a | 1.25 ± 1.35 b | 2.02 ± 2.48 a |
| Glycine | 1.56 ± 0.28 a | 1.68 ± 0.41 a | 1.83 ± 0.28 b | 1.18 ± 1.16 b | 1.88 ± 1.62 a | 2.02 ± 1.86 a |
| Arginine | 0.31 ± 0.1 b | 0.35 ± 0.15 b | 0.37 ± 0.17 b | 0.88 ± 0.78 a | 0.84 ± 0.81 a | 0.41 ± 0.61 a |
| Alanine | 0.58 ± 0.1 a | 0.71 ± 0.03 a | 0.68 ± 0.12 a | 0.51 ± 0.5 a | 0.57 ± 0.5 a | 0.65 ± 0.61 a |
| Proline | 1.19 ± 0.44 b | 1.34 ± 0.46 b | 1.59 ± 0.59 b | 2.67 ± 3.11 a | 2.37 ± 3.26 a | 3.09 ± 3.42 a |
| Histidine | 0.74 ± 0.11 a | 0.81 ± 0.11 a | 0.89 ± 0.11 a | 0.58 ± 0.58 b | 0.41 ± 0.59 b | 0.52 ± 0.74 b |
| Valine | 0.89 ± 0.38 a | 0.97 ± 0.43 a | 1.05 ± 0.48 a | 0.79 ± 0.54 a | 0.65 ± 0.55 b | 0.96 ± 0.71 b |
| Methionine | 0.47 ± 0 b | 0.55 ± 0 b | 0.63 ± 0 b | 0.85 ± 0.89 a | 0.86 ± 0.89 a | 0.86 ± 0.89 a |
| Cystine | 0.87 ± 0.06 b | 0.89 ± 0.03 b | 0.92 ± 0.06 b | 1.44 ± 1.17 a | 1.45 ± 1.26 a | 1.41 ± 1.37 a |
| Isoleucine | 1.11 ± 0.72 b | 1.12 ± 0.76 b | 1.14 ± 0.8 b | 1.39 ± 1.13 a | 1.46 ± 1.17 a | 1.28 ± 0.95 a |
| Leucine | 1.04 ± 0.15 a | 1.01 ± 0.29 a | 0.98 ± 0.43 b | 1.02 ± 0.86 a | 1.15 ± 0.87 a | 1.34 ± 1 a |
| Phenylalanine | 1.98 ± 1.08 a | 1.82 ± 1.17 a | 1.65 ± 1.45 b | 1.88 ± 2.14 a | 1.13 ± 2.11 b | 2.27 ± 2.44 a |
| Tyrosine | 0.31 ± 0.08 b | 0.32 ± 0.05 b | 0.33 ± 0.05 b | 0.4 ± 0.35 a | 0.44 ± 0.34 a | 0.47 ± 0.41 a |
| Lysine | 0.77 ± 0.14 b | 0.84 ± 0.1 b | 0.92 ± 0.07 a | 1.07 ± 0.92 a | 1 ± 0.9 a | 0.85 ± 0.82 b |
| Threonine | 1.33 ± 0.28 a | 1.39 ± 0.26 a | 1.45 ± 0.24 a | 1.47 ± 1.25 a | 1.23 ± 1.26 a | 1.59 ± 1.46 a |
| Tryptophan | 0.78 ± 0.21 b | 0.84 ± 0.23 b | 0.9 ± 0.24 b | 1.11 ± 0.92 a | 1.32 ± 1.04 a | 1.36 ± 1.1 a |
| Nitrogen content and metabolism | ||||||
| Nitrogen (g/100 g) | 23.39 ± 0.89 b | 19.69 ± 0.82 b | 15.72 ± 0.53 b | 28.11 ± 1.24 a | 24.95 ± 0.72 a | 19.20 ± 0.8 a |
| Total protein (mg/g FW) | 169.5 ± 1.9 a | 153.61 ± 8.86 a | 118.0 ± 3.16 b | 99.6 ± 2.27 b | 129.0 ± 8.47 b | 136.8 ± 2.81 a |
| Nitrate reductase μmol nitrite/mg protein.min |
45.24 ± 0.03 a | 49.53 ± 2.47 b | 86.19 ± 5.45 b | 33.11 ± 2.23 b | 56.36 ± 0.91 a | 118 ± 11.27 a |
| GDH μmol NADH/mg protein.min |
4.14 ± 0.21 a | 4.10 ± 0.09 a | 6.99 ± 0.48 b | 4.14 ± 0.21 a | 4.10 ± 0.09 a | 10.33 ± 0.48 a |
| GOGAT μmol NADH/mg protein.min |
7.83 ± 0.28 a | 11.16 ± 0.45 b | 14.35 ± 0.45 b | 6.35 ± 0.29 b | 14.70 ± 0.52 a | 21.38 ± 1.83 a |
| GS μmol γ-glutamyl hydroxamate/mg protein.min |
16.12 ± 0.91 b | 23.09 ± 1.24 b | 26.16 ± 0.45 b | 23.00 ± 1.24 a | 29.2 ± 0.77 a | 32.55 ± 0.8 a |