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. 2021 Dec 29;14(1):151. doi: 10.3390/nu14010151

Figure 3.

Figure 3

Chromatography of fraction B2 separated by RP-HPLC (Reverse high performance liquid chromatography) on a Shimadzu C18 column (5 μm, 4.6 × 250 mm) using a linear gradient of acetonitrile (20–100% in 0–25 min) in 0.1% TFA at a flow rate of 1.0 mL/min (A). Fractions P1-P10 (B). ACE inhibition activity of fractions P1-P10 (C) were separately isolated by analytical RP-HPLC. Control: Glutelin-1 hydrolysate before separation and purification. ** p < 0.01, vs. the control. P1–P10 represent 10 major fractions obtained after separation by RP-HPLC.