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Components of amino acid-free medium (ZERO medium)
| Variable | Value, mg/L |
|---|---|
| CaCl2 • 9H2O | 265 |
| Fe(NO3)3 • 9H2O | 0.1 |
| KCl | 400 |
| MgSO4 (anhyd) | 97.67 |
| NaCl | 4,750 |
| NaHCO3 | - |
| NaH2PO4 | 109 |
| D-Glucose | - |
| Phenol red | 14.93 |
| Sodium pyrubate | - |
| HEPES | 5,958 |
| D-Ca pantothenate | 4 |
| Choline chloride | 4 |
| Folic acid | 4 |
| I-inositol | 7.2 |
| Niacinamide | 4 |
| Riboflavin | 0.4 |
| Thiamine HCl | 4 |
| Pyridoxine HCl | 4 |
HEPES, hydroxyethyl piperazine ethane sulfonicacid.
