Table 2. ctDNA status based on characteristics for all plasma samples (N=258).

	ctDNA					P value
	Positive			Negative
	N	%		N	%
Total	67	26		191	74
Gender
Male	46	30		105	70	0.0505
Female	21	20		86	80
Stage
I	6	21		23	79	0.00356
II	11	18		50	82
III	14	20		55	80
IV	26	44		33	56
Ethnicity
Caucasian	35	25		106	75	0.14
Asian	16	30		38	70
Hispanic	6	19		26	81
AA	8	44		10	56
Other	0	0		12	100
Tumor grade
Low	4	17		20	83	0.17
Moderate	24	28		63	72
High	19	37		32	63
Tumor type
GEC	7	17		34	83	0.3
Pancreas	11	30		26	70
Hepatobiliary	6	32		13	68
Small bowel	1	17		5	83
Appendix	4	25		12	75
Colon	27	26		77	74
Rectum	9	27		24	73
Anal	2	100		0	0
MSI-high
Yes	5	42		7	58	0.45
No	22	31		50	69
Systemic treatment
Yes	24	31		53	69	0.09
No	36	21		134	79
Site of metastasis
Liver	10	37		17	63	0.04
Lung	8	73		3	27
Peritoneum	10	53		9	47
Other	7	26		20	74
No. of metastatic sites
0	31	22		142	78	<0.0001
1	17	30		39	70
2+	19	66		10	34

AA, African American; GEC, gastric/esophageal carcinomas; due to rounding, percentages might not exactly add up to 100%.