Figure 5.

JMJD3 regulated Aβ and MSR through BDNF. The effects of JMJD3 RNAi and JMJD3 RNAi+BNDF on protein levels of JMJD3 and BNDF were analyzed by WB (A, B). Their effects on Aβ levels were analyzed by WB (C). Their effects on cell proliferation were analyzed by CCK-8 assay (D). Their effects on apoptosis were analyzed by Flow cytometry (E). Their effects on MSR markers were analyzed by WB (F-H). Their effects on H3K27me3 levels at the BDNF promoter region were analyzed by ChIP-qPCR (I). Note: *P<0.05, **P<0.01 vs. AAV-NC group, #P<0.05 vs. JMJD3 RNAi.