Silencing of USP7 inhibited the proliferation and migration of HCC cells. A: CCK-8 assay showed that USP7 expression was down-regulated in Hep3B cells, and cell proliferation was reduced (n=3); CCK-8 assay showed that USP7 expression was down-regulated in HUH7 cells, and cell proliferation was reduced (n=3); B: EdU assay showed that USP7 expression was down-regulated in Hep3B and HUH7 cells, and cell proliferation was reduced (Magnification: 200×; n=3); C: Transwell assay showed that USP7 expression was down-regulated in Hep3B and HUH7 cells, and cell migration was reduced (Magnification: 200×; n=3); D: Transwell assay showed that USP7 expression was down-regulated in Hep3B and HUH7 cells, and cell invasion ability was weakened (Magnification: 200×; n=3). Compared with NC group cells, *P<0.05; compared with NC group cells, **P<0.01.