Table 2.

Multilocus sequence typing

Locus	Primers (specific amplification)	Amplicon size	Polymorphisms
SOD	F1: AATAAGGGTTTAATTAGTCTT	762 bp	110, 191, 215
	R1: CTTACATTCCATATATTTTCA	670 bp
	F2: GAACCTTATCTTTCTCAT
	R2: TCATTTTAGTATTTAGTCTC
mtLSU rRNA	F1: GTGAAAGCCCAGAGTCCC	1216 bp	54-57, 80, 85, 248, 288
	R1: CCGAGTTCCTTCGCAATA	738 bp
	F2: CTTTTGCATAATGGGTCAG
	R2: TTTTCGGCGAATAGGATTT
CYB	F1: TATGGTTCATTATCAGGACTG	1016 bp	279, 299, 348, 362, 369, 516, 547, 566, 675, 742, 832-833, 838
	R1: ACCAGAGGAATAACAACTAAG	942 bp
	F2: ACAGATTATTACGGGTGT
	R2: GTAGCATATTGTCCAAGC

SOD, the superoxide dismutase; mtLSU rRNA, mitochondrial large subunit of ribosomal RNA; CYB, cytochrome b.