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. 2021 Dec 23;19(1):81–89. doi: 10.1038/s41592-021-01328-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — Culture medium with or without supplementation with the natural CXCR4 ligand SDF-1-α was placed in the bottom chamber of a trans-well and CD4⁺ T cells (WT or CXCR4 KO) were added into the top chamber. After three hours, the absolute number of cells in the bottom chamber was quantified by flow cytometry using BD Trucount™ Tubes (schematic created with BioRender.com).