Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 16;19(1):65–70. doi: 10.1038/s41592-021-01341-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 5 — a-e The activity of the GTPase RhoA as well as the cell cycle stage were followed over time for dividing cells. FLIM images are given for both Raichu-RhoA-CR and LT-Fucci(CA) labeled with MaP618-CA (1 μm) along with a transmission light image and the phasor plot 1 h 20 min before cytokinesis (a), 30 min before cytokinesis (b), shortly after cytokinesis (c), and 1 h 20 min after cytokinesis (d). The FLIM images are color-coded according to the scales in (e). The two biosensors could be easily multiplexed measuring fluorescence lifetime in the GFP channel (Clover-Raichu-RhoA-CR) and the CPY channel (LT-Fucci(CA)-MaP618). As expected mitotic cells initially showed low RhoA activity indicated by long donor fluorescence lifetimes (2.7 ns). However, closer to cytokinesis RhoA activity started to increase (2.6 ns) until a donor fluorescence lifetime of 2.5 ns was reached directly after cytokinesis. After division the cells reached even higher RhoA activity (2.2 ns)⁶⁹. Scale bar, 10 μm.