FIGURE 8.

Thr50‐dependent is required for FBXW7‐mediated degradation of MLST8. A, Comparison of FBXW7 binding sites in MLST8 with the FBXW7‐binding consensus motif defined in the known FBXW7 substrates. B, Conserved CPD motifs in MLST8 in different species. C, Western blotting analysis of co‐immunoprecipitation of ectopically expressed FLAG‐MLST8 WT, FLAG‐MLST8 T50A/S54A, and Myc‐FBW7α in 293T cells. D, 786‐O cells were transfected with the indicated plasmids for 24 h, followed by Western blotting analysis. E and F, 786‐O cells were transfected with the indicated plasmids. After 24 h, cells were treated with 50 μg/mL CHX. G, 786‐O cells were transfected with the indicated plasmids for 16 h, followed by treatment with 20 μm MG132 for 8 h