TABLE 1.
Sequences of primers for sgRNAs
| Gene name | Sequences of siRNAs |
|---|---|
| sgControl | Forward: 5′CACCGGGGCAGGTCCACAATACTAC3′ |
| Reverse: 5′AAACGTAGTATTGTGGACCTGCCCC3′ | |
| sgFBXW7#1 | Forward: 5′CACCGTTCGGCGTCGTTGTTGCCCT3′ |
| Reverse: 5′AAACAGGGCAACAACGACGCCGAAC3′ | |
| sgFBXW7#2 | Forward: 5′ CACCGAGTGGAAGTATGCCCATATA3′ |
| Reverse: 5′ AAACTATATGGGCATACTTCCACTC3′ | |
| sgMLST8#1 | Forward: 5′CACCGGACGGCCGCTGGATGTACAC3′ |
| Reverse: 5′ AAACGTGTACATCCAGCGGCCGTCC3′ | |
| sgMLST8#2 | Forward: 5′CACCGAGACGGCCGCTGGATGTACA3′ |
| Reverse: 5′ AAACTGTACATCCAGCGGCCGTCTC3′ | |
| siRBX1#1 | Forward: 5′GAAGCGCTTTGAAGTGAAA3′ |
| Reverse: 5′TTTCACTTCAAAGCGCTTC3′ | |
| siRBX1#2 | Forward: 5′GGGATATTGTGGTTGATAA3′ |
| Reverse: 5′TTATCAACCACAATATCCC3′ | |
| siRBX1#3 | Forward: 5′GGAACCACATTATGGATCT3′ |
| Reverse: 5′AGATCCATAATGTGGTTCC3′ | |
| siCUL1#1 | Forward: 5′CAACGAAGAGTTCAGGTTT3′ |
| Reverse: 5′AAACCTGAACTCTTCGTTG3′ | |
| siCUL1#2 | Forward: 5′CGAGGAAGACCGCAAACTA3′ |
| Reverse: 5′TAGTTTGCGGTCTTCCTCG3′ | |
| siCUL1#3 | Forward: 5′AGACAGTGCTTGATGTTCA3′ |
| Reverse: 5′TGAACATCAAGCACTGTCT3′ | |
| siCUL2#1 | Forward: 5′GGAAGTGCATGGTAAATTT3′ |
| Reverse: 5′AAATTTACCATGCACTTCC3′ | |
| siCUL2#2 | Forward: 5′CATCCAAGTTCATATACTA3′ |
| Reverse: 5′TAGTATATGAACTTGGATG3′ | |
| siCUL2#3 | Forward: 5′GCAGAAAGACACACCACAA3′ |
| Reverse: 5′TTGTGGTGTGTCTTTCTGC3′ | |
| siCUL3#1 | Forward: 5′GAGAAGATGTACTAAATTC3′ |
| Reverse: 5′GAATTTAGTACATCTTCTC3′ | |
| siCUL3#2 | Forward: 5′CGACAGAAAACATGAGATA3′ |
| Reverse: 5′TATCTCATGTTTTCTGTCG3′ | |
| siCUL3#3 | Forward: 5′GAAAGTAGACGACGACAGA3′ |
| Reverse: 5′TCTGTCGTCGTCTACTTTC3′ | |
| siCUL4A#1 | Forward: 5′GCACAGATCCTTCCGTTTA3′ |
| Reverse: 5′TAAACGGAAGGATCTGTGC3′ | |
| siCUL4A#2 | Forward: 5′GAACAGCGATCGTAATCAA3′ |
| Reverse: 5′TTGATTACGATCGCTGTTC3′ | |
| siCUL4A#3 | Forward: 5′GCATGTGGATTCAAAGTTA3′ |
| Reverse: 5′TAACTTTGAATCCACATGC3′ | |
| siCUL4B#1 | Forward: 5′TAAATAACCTCCTTGATGA3′ |
| Reverse: 5′TCATCAAGGAGGTTATTTA3′ | |
| siCUL4B#2 | Forward: 5′CAGAAGTCATTAATTGCTA3′ |
| Reverse: 5′TAGCAATTAATGACTTCTG3′ | |
| siCUL4B#3 | Forward: 5′CGGAAAGAGTGCATCTGTA3′ |
| Reverse: 5′TACAGATGCACTCTTTCCG3′ | |
| siCUL5#1 | Forward: 5′GACACGACGTCTTATATTA3′ |
| Reverse: 5′TAATATAAGACGTCGTGTC3′ | |
| siCUL5#2 | Forward: 5′GCAAATAGAGTGGCTAATA3′ |
| Reverse: 5′TATTAGCCACTCTATTTGC3′ | |
| siCUL5#3 | Forward: 5′TAAACAAGCTTGCTAGAAT3′ |
| Reverse: 5′ATTCTAGCAAGCTTGTTTA3′ |