FIGURE 3.

CircUSP34 acts as a sponge to regulate miR‐16‐5p. A, The potential target site between circUSP34 and miR‐16‐5p was predicted by Starbase. B, The cellular location of circUSP34 (green) and miR‐16‐5p (red) in cells was observed by performing FISH (magnification: 400×, scale bar: 100 μm). C, miR‐16‐5p expression level was confirmed by qRT‐PCR after transfecting sh‐circUSP34. D, The relative luciferase activity was detected in the KHOS cells after cotransfecting with circUSP34‐WT or circUSP34‐MUT and mimics or NC, respectively. E, RNA immunoprecipitation (RIP) assay was performed in KHOS cells after transfecting with miR‐16‐5p mimic or NC respectively, with qRT‐PCR and nucleic acid electrophoresis. F, The enrichment of circTLK1 was measured by qRT‐PCR in RNA pull down assay. Data are shown as mean ± SD. ns, no significance;*P < 0.05, **P < 0.01