FIGURE 6.

CircUSP34 promotes proliferation and metastasis of OS in vivo. A, Subcutaneous tumors of circUSP34‐knockout and NC. B, Tumor weight was measured in the sh‐circUSP34 and sh‐NC groups which were analyzed. C, Tumor volume was measured every week. D, Relative expression level of miR‐16‐5p was quantified in tumor tissues by qRT‐PCR. E, Representative images of HE staining were exhibited from the sh‐circUSP34 group and sh‐NC group. F, The expression of Vimentin, Ki‐67, N‐cadherin, and E‐cadherin was evaluated by performing IHC. G, Western blot assay showed the protein expression level of Vimentin, Ki‐67, N‐cadherin, and E‐cadherin from tumor tissue. Data are shown as mean ± SD. *P < 0.05, **P < 0.01