FIGURE 2.

Bromodomain‐containing protein 4 inhibitors decrease the expression of PD‐L1 in liver cancer. A, L02 cells were treated with JQ1 (0, 0.625, 1.25, 2.5, 5, 10, 20, 40, 80, or 160 μmol/L) and CPI‐203 (0, 0.625, 1.25, 2.5, 5, 10, 20, 40, 80, or 160 μmol/L) for 48 h; proliferation was detected using MTT. B, Cells were seeded into six‐well plates, and cultured in different concentrations of CPI‐203 for about 2 wk. The clones were scanned using an enzyme‐linked immunodot analyzer. C, CPI‐203 was administrated in vivo, the tumor tissue single cells were stained by antibody, the percentage expression of PD‐L1 was detected by FCM (*P < .05)