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. 2021 Dec 2;113(1):170–181. doi: 10.1111/cas.15182

FIGURE 6.

FIGURE 6

The TDO2‐AHR pathway mediates the maintenance of Wnt signaling in colon cancer spheroids. A, qPCR analyses of representative Wnt target genes in the metastatic spheroids (CRC‐24 M) after treatment with the indicated concentrations of the inhibitor of TDO2 or AHR for 96 h. B, Immunostaining of the spheroids (CRC‐24 M) with β‐catenin in the presence of the indicated inhibitor for 96 h. Nuclei were counterstained with DAPI. Relative quantification values of nuclear β‐catenin were shown in the bottom columns. Scale bar: 25 μm. C, qPCR analyses of representative Wnt target genes in the AHR‐ or TDO2‐inhibited spheroids shown in Figure 5G (100 μmol/L). D, Immunostaining of the spheroids shown in Figure 5I. Relative quantification values of nuclear β‐catenin were shown in the bottom columns. Scale bar: 25 μm. Values represent the mean ± SD; *P < .05, **P < .01, ***P < .001